Convergent synthesis of ribonuclease L-active 2',5'-oligoadenylate-peptide nucleic acids

Z Wang; L Chen; S F Bayly; P F Torrence

doi:10.1016/s0960-894x(00)00240-7

Convergent synthesis of ribonuclease L-active 2',5'-oligoadenylate-peptide nucleic acids

Bioorg Med Chem Lett. 2000 Jun 19;10(12):1357-60. doi: 10.1016/s0960-894x(00)00240-7.

Authors

Z Wang¹, L Chen, S F Bayly, P F Torrence

Affiliation

¹ Section on Biomedical Chemistry, Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive antd Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0805, USA.

PMID: 10890163
DOI: 10.1016/s0960-894x(00)00240-7

Abstract

2-5A was conjugated to N-(2-aminoethyl)-glycyl PNA by periodate oxidization, followed by coupling with amino-derivatized PNA and final cyanoborohydride reduction. An adduct of 2-5A pentamer with tetrameric thymine PNA activated RNase L with the same potency as earlier versions of 2-5A-PNA or 2-5A-DNA.

MeSH terms

Adenine Nucleotides / chemistry*
Endoribonucleases / metabolism*
Oligoribonucleotides / chemistry*
Peptide Nucleic Acids / chemical synthesis*
Peptide Nucleic Acids / chemistry
Peptide Nucleic Acids / metabolism

Substances

Adenine Nucleotides
Oligoribonucleotides
Peptide Nucleic Acids
2',5'-oligoadenylate
Endoribonucleases
2-5A-dependent ribonuclease